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How to determine dwell volume of an HPLC or UHPLC system

Determining the dwell volume of an LC system is very useful as it is the basis of gradient elution method transfer.

Understanding the Relationship Between Particle Size, Performance and Pressure

Have you ever been confused as to the best solution to a certain separation problem? We explain the effect of these parameters on performance.

The Power of Stationary Phase Selectivity

One of the key variables in you method development is stationary phase selectivity. You can learn more about this important tool.

A Simple Step-by-Step Protocol for HILIC Method Development

If you are looking for a clear introduction to the practicalities of HILIC, this is a comprehensive guide for those unfamiliar with this mode of HPLC.

Creating the Correct Environment for Analysing Reducing Sugars

How you can create the correct environment for the analysis of monosaccarides and reducing disaccharides

The Importance of Sample Diluent in HILIC

Your choice of sample diluent in HILIC can have a profound effect on the chromatography.  This note explains why.

Column Equilibration in HILIC Mode

It is essential to fully equilibrate your HILIC columns prior to analysis to ensure a stable adsorbed water layer