Lipid rafts are membrane microdomains that are enriched in caveolin, cholesterol, glycolipids, sphingolipids and glycosyl-phosphatidylinositol. Lipid rafts are also known as detergent-insoluble glycolipid-enriched complexes (GEMs) or DIGs. Many signalling proteins, including glycosylphosphatidylinositol (GPI)-anchored proteins, doubly-acylated tyrosine kinases of the Src family, and transmembrane proteins, are located in lipid rafts. Lipid raft localised proteins have been shown to be involved in intracellular membrane trafficking and signalling.
- Fractionate lipid raft localised proteins
- A 2D electrophoresis compatible buffer is provided
FOCUS™ Signal Proteins kit fractionates lipid raft localised proteins from other cellular proteins by employing non ionic detergents. Signal Protein Extraction Buffer is a proprietary formulation of non-ionic detergents designed to efficiently extract and remove soluble proteins, leaving lipid rafts containing signal proteins as a detergent insoluble fraction. The resulting rafts are then solubilised in FOCUS™ Protein Solubilisation Buffer, a supplied 2D electrophoresis compatible buffer or a different buffer of choice.
FOCUS™ Signal Proteins kit is designed for >50 preps, where one prep is either 100 mg mammalian tissue, 50 µl wet animal cell pellet; 50 µl wet yeast pellet; 50 µl wet bacteria pellet or 250 mg plant tissue.