NGF Rapid™ ELISA kit: human

Assay Type:
Sandwich
Format:
Pre-coated
Primary antibody reactivity:
Human
Target protein:
NGF
Cross reactivity:
The antibodies used in this ELISA kit bind epitopes within the mature domain of the protein and therefore can recognise both the pro- and the mature form of NGF. However, human proNGF protein shows <0,1% cross-reactivity (determined at 25 ng/ml) allowing specific quantification of mature NGF. This NGF ELISA does not cross-react with brain derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), NT-4/5, glial cell line-derived neurotrophic factor (GDNF) and vascular endothelial growth factor (VEGF165) tested at 25 ng/ml. Due to a high degree of NGF sequence homology, the antibodies used in this kit will also detect NGF from other species including mouse and rat.
Detection range:
3,9 – 250 pg/ml
Sensitivity:
Typical limit of detection (LOD) for human NGF is 2 pg/ml determined as 150% of the blank value