17-phenyl trinor prostaglandin F2α ELISA kit

Leverandør: Cayman Chemical
CAYM516821-96EA 9325 NOK
CAYM516821-96 CAYM516821-96S CAYM516821-480S CAYM516821-480
17-phenyl trinor prostaglandin F2α ELISA kit
Assay ELISAs
A sensitive detection method for measuring both the free acid and ethyl amide forms of 17-phenyl trinor PGF.

  • Measure 17-phenyl trinor PGF (also known as bimatoprost)
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Measure 17-phenyl trinor PGF levels down to 2,6 pg/ml
  • Colorimetric reading at 405 to 420 nm

17-phenyl trinor Prostaglandin F (17-phenyl trinor PGF) is a metabolically stable analog of PGFand is a potent agonist for the FP receptor. It binds to the FP receptor on ovine luteal cells with a relative potency of 756% compared to that of PGF. At the rat recombinant FP receptor expressed in CHO cells, 17-phenyl trinor PGF inhibits PGF binding with a KI value of 1,1 nM. In human and animal models of glaucoma, FP receptor agonist activity corresponds very closely with intraocular hypotensive activity. 17-phenyl trinor PGFethyl amide is an F-series prostaglandin analog which has been approved for use as an ocular hypotensive drug, sold under the Allergan trade name Bimatoprost. Whether 17-phenyl trinor PGF ethyl amide is a prodrug analogous to prostaglandin ester prodrugs such as latanoprost is currently controversial. There is some evidence that unmetabolized 17-phenyl trinor PGFis a weak FP receptor agonist. 17-phenyl trinor PGF is converted by an amidase enzymatic activity in the bovine and human cornea to yield the corresponding free acid, with a conversion rate of about 40 µg/g corneal tissue/24 hours. Cayman’s 17-phenyl trinor PGF ELISA is a sensitive detection method for measuring both the free acid and ethyl amide forms of 17-phenyl trinor PGF. The assay is most appropriate for use when only one of the two forms is present. Samples containing mixtures of both the ethyl amide and free acid should be purified and the two compounds separated prior to ELISA analysis.

Leveringsinformasjon: Kit contains antiserum, tracer, standards, ELISA and wash buffer concentrate; polysorbate 20, plate and cover sheet, Ellman's reagent, tracer and antiserum dye.
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