The Antibody column package includes modern protein A affinity and size exclusion chromatography columns to obtain highly pure human IgG.
- Contains columns suitable for a successful 2-step protocol of human antibodies: HiTrap MabSelect PrismA (1×1 ml) and HiPrep 16/60 Sephacryl S-300 HR
- Includes instruction with guidance and tips to help you quickly get started
HiTrap MabSelect PrismA column is prepacked with MabSelect PrismA resin for preparative purification of monoclonal and polyclonal antibodies from serum and cell culture supernatant, providing excellent purity in one step. Obtain final high purity with the HiPrep 16/60 Sephacryl S-300 HR size exclusion column, which removes antibody aggregates and/or fragments.
MabSelect PrismA resin containing a protein A-derived ligand produced in E. coli. The ligand has been specially engineered to create an affinity resin with enhanced alkali and protease stability. The specificity of binding to the Fc region of IgG is similar to conventional Protein A and provides you with excellent purification in one step.
HiPrep 16/60 Sephacryl S-300 HR size exclusion chromatography column is prepacked with Sephacryl S-300 HR chromatography resin, which has a broad fractionation range of Mr 10000 to 1500000 for globular proteins. The resin is well-suited for antibody purification and the high sample capacity of the HiPrep column.