RPMI 1640 (1X), Cell Culture Media, MP Biomedicals
RPMI has successfully been used for the cultivation of normal human and neoplastic leukocytes. It is now a popular general purpose medium when properly supplemented. As a modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent.
- Originally developed by Moore and his colleagues at Roswell Park Memorial Institute (RPMI)
- Widely used in fusion protocols and in the growth of hybrid cells
- Used with a serum supplement, to support several cell lines in the absence of serum
It was based on the RPMI 1630 line of media which utilised a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins.
The Dutch Modification of RPMI 1640 is also available through MP, contains 6400 mg/L sodium chloride instead of 6000 mg/L, 1000 mg/L sodium bicarbonate instead of 2000 mg/L, and 20 mM HEPES.
- Storage temperature:4 °C
- Media FormatPk
- RPMI 1640 (1X Solution) without L-Glutamine and L-LeucineRPMI 1640 (1X Solution) without L-Glutamine and L-LeucineICNA091629149091629149Liquid100 mL1 * 100 mLkr 940,00
- 1X RPMI without L-Glutamine, L-Cysteine, L-Cystine, and L-Methionine1X RPMI without L-Glutamine, L-Cysteine, L-Cystine, and L-MethionineICNA091646454091646454Liquid500 mL1 * 500 mLkr 700,00