His tag protein purification by IMAC is both popular and highly efficient. HisTrap HP columns are packed with Ni Sepharose High Performance (HP) affinity resin. This resin consists of highly cross-linked agarose beads to which a chelating group has been coupled. The chelating group is precharged with nickel, which selectively retains proteins with exposed histidine groups.
HisTrap HP IMAC resin has a binding capacity that exceeds 40 mg/ml, resulting in high protein recovery. The small bead size (34 µm) contributes to a purer purification compared to nickel resins of larger bead size.