IPG buffers are ampholyte-containing buffer concentrates specifically formulated for use with Immobiline™ DryStrip gels for isoelectric focusing, run as the first dimension of 2-D electrophoresis or as a separate application. Using Immobiline DryStrip gels together with matching IPG buffer gives high sample loading capacity, improves resolution, and increases the probability of detecting low abundance proteins.
- For use with Immobiline DryStrip gels to improve protein solubility
- Eliminate background staining
- Ensure optimal conditions for IEF separation
IPG buffers pH 3 to 10 and pH 3 to 10 NL offer improved resolution, sensitivity, and increased spot intensity, as well as additional protein spots in high molecular weight and basic pH areas
Each IPG buffer produces uniform conductivity along the Immobiline™ DryStrip during focusing, resulting in wider latitude in run times. IPG buffers also eliminate high background staining. IPG buffers are supplied in 1 ml aliquots of water. The buffer is diluted 50- or 200-fold in the rehydration solution, depending on the first-dimension system and pH range of the strip.
Bestillingsinformasjon: Specification supplied with the buffer.
NB: Estimated shelf life from manufacture date: 36 months