Affinity chromatography media, Glutathione Sepharose™ 4 Fast Flow

Leverandør: Cytiva
Sepharose™
17-5132-02EA 24175 NOK
17-5132-02 17-5132-01 17-5132-03
Affinity chromatography media, Glutathione Sepharose™ 4 Fast Flow
Proteinrense Protein Purification Resins
Glutathione Sepharose 4 Fast Flow is used for the purification of GST-tagged proteins from bacterial lysates and other S-tranferases or glutathione-dependent proteins.

  • For affinity chromatography
  • Fast, one-step purification of GST-tagged proteins
  • Mild elution conditions preserving protein antigenicity and function
  • For scale-up due to high flow properties
  • Glutathione coupled to Sepharose 4 Fast Flow via a 10-carbon spacer arm
  • Epoxy activation coupling method
  • Stable in all commonly used aqueous buffers

Mild elution conditions preserve protein antigenicity and function. Sepharose 4 Fast Flow provides good flow properties and is excellent for scaling up. Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.
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