About this item
qScript™ cDNA synthesis kit contains qScript™ Reverse Transcriptase, a mixture of a MMLV reverse transcriptase derivative and ribonuclease inhibitor protein, optimised for sensitive and reliable cDNA synthesis over a broad dynamic range of input RNA. cDNA synthesis reagents are provided in a variety of SuperMix and kit formats to suit specific applications.
- Supermix format ideal for high throughput applications
- Unbiased representation gives confidence that RNA is represented accurately in the resulting cDNA
- Broad dynamic range ensures data from precious samples is more reliably attained
- Choice of priming methods to suit individual needs
qScript™ cDNA SuperMix is an easy to use solution for two-step RT-PCR. This one-tube supermix contains all components for cDNA synthesis including buffer, dNTPs, MgCl₂, primers, RNase inhibitor, qScript™ Reverse Transcriptase and stabilisers. Only RNA needs to be added. Complete cDNA synthesis is achieved in 40 minutes. Consistency and reproducibility are achieved over a broad dynamic range regardless of RNA input. In addition, qScript™ cDNA SuperMix offers excellent sensitivity and accurate representation of low abundance genes. The supermix format eliminates multiple component additions providing exceptional reproducibility and precision. qScript™ cDNA SuperMix is available in both single tube or 96-well format.
qScript™ cDNA Synthesis Kit is a sensitive and easy to use kit for RNA quantification using two-step RT-PCR. This kit contains an optimised blend of random and oligo (dT) primers for robust, consistent and unbiased first-strand synthesis over a broad range of RNA template concentrations. The qScript™ Reaction Mix (5X) contains all required reagents for cDNA synthesis, except for qScript™ Reverse Transcriptase and RNA. qScript™ Reverse Transcriptase is provided in a separate tube. This kit is intended for use by researchers using Bio-Rad iScript™ cDNA Synthesis Kit for ongoing studies or established Standard Operating Procedures that cannot readily be switched to qScript™ cDNA SuperMix.
qScript™ Flex cDNA Kit is an easy to use format for first-strand cDNA synthesis that supports multiple RNA priming strategies. The qScript™ Flex reaction mix (5X) is optimised for use with oligo dT, random or gene specific primers in any combination. A proprietary cDNA-priming enhancer that improves sensitivity is provided pre-mixed with random and oligo dT primers, and in a separate tube for use with gene specific primers (user provided). qScript™ Reverse Transcriptase is provided in a separate tube.
qScript™ Flex cDNA kit is an easy to use format for first-strand cDNA synthesis that supports multiple RNA priming strategies. The qScript™ Flex reaction mix (5X) is optimised for use with oligo dT, random or gene-specific primers in any combination. A proprietary cDNA-priming enhancer that improves sensitivity is provided pre-mixed with random and oligo dT primers, and in a separate tube for use with gene-specific primers (user provided). qScript™ Reverse Transcriptase is provided in a separate tube.
Product Information
- Catalog #Supplier #PkAvailabilityPrice
- qScript™ cDNA SuperMix, 25 reactionsqScript™ cDNA SuperMix, 25 reactions733-117695048-02525 AssaysLog in for availability1 * 1 Eachkr 2 850,00Supplier Article Number: 95048-025Local Article Number: QUNT95048-025
- qScript™ cDNA SuperMix, 100 reactionsqScript™ cDNA SuperMix, 100 reactions733-117795048-100100 AssaysLog in for availability1 * 1 Eachkr 11 100,00Supplier Article Number: 95048-100Local Article Number: QUNT95048-100
- qScript™ cDNA SuperMix, 500 reactionsqScript™ cDNA SuperMix, 500 reactions733-117895048-500500 AssaysLog in for availability1 * 1 Eachkr 42 500,00Supplier Article Number: 95048-500Local Article Number: QUNT95048-500
- qScript™ cDNA Synthesis Kit, 25 reactionsqScript™ cDNA Synthesis Kit, 25 reactions733-117395047-02525 AssaysLog in for availability1 * 1 Eachkr 2 400,00Supplier Article Number: 95047-025Local Article Number: QUNT95047-025
- qScript™ cDNA Synthesis Kit, 100 reactionsqScript™ cDNA Synthesis Kit, 100 reactions733-117495047-100100 AssaysLog in for availability1 * 1 Eachkr 8 425,00Supplier Article Number: 95047-100Local Article Number: QUNT95047-100
- qScript™ cDNA Synthesis Kit, 500 reactionsqScript™ cDNA Synthesis Kit, 500 reactions733-117595047-500500 AssaysLog in for availability1 * 1 Eachkr 34 700,00Supplier Article Number: 95047-500Local Article Number: QUNT95047-500
- qScript™ Flex cDNA Kit, 25 reactionsqScript™ Flex cDNA Kit, 25 reactions733-118095049-02525 AssaysLog in for availability1 * 1 Eachkr 2 875,00Supplier Article Number: 95049-025Local Article Number: QUNT95049-025
- qScript™ Flex cDNA Kit, 100 reactionsqScript™ Flex cDNA Kit, 100 reactions733-118195049-100100 AssaysLog in for availability1 * 1 Eachkr 9 975,00Supplier Article Number: 95049-100Local Article Number: QUNT95049-100