His Mag Sepharose® excel is a magnetic immobilised metal ion affinity chromatography (IMAC) medium pre-charged with nickel ions that are strongly bound to a chelating ligand. The medium enables small scale capture, purification and reproducible screening of histidine-tagged proteins expressed and secreted into cell culture supernatants from eukaryotic cells such as insect cells or Chinese Hamster Ovary (CHO) cells.
- Increased target protein yield
- Minimised degradation through reduced and simplified sample handling
- Simplify handling using visible and dense Sepharose-based magnetic beads
The Mag Sepharose® format has excellent properties for small scale experiments. The high density of the beads allows rapid capture by magnetic devices while the visibility of the beads ensures reliable collection of the bound histidine-tagged proteins during the purification procedure. The base matrix is based on Sepharose® medium with magnetite incorporated.